Reporter

Part:BBa_K1676075:Design

Designed by: Ooi Kean Hean   Group: iGEM15_NTU-Singapore   (2015-09-18)


GFP Reporter with Mutant RBS Opt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


Design Notes

Primers were designed to contain desired mutations for Quickchange PCR


Source

Site targeted mutagenesis was carried out on original RBS , BBa_B0034

References