Reporter
Part:BBa_K1676075:Design
Designed by: Ooi Kean Hean Group: iGEM15_NTU-Singapore (2015-09-18)
GFP Reporter with Mutant RBS Opt
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 725
Design Notes
Primers were designed to contain desired mutations for Quickchange PCR
Source
Site targeted mutagenesis was carried out on original RBS , BBa_B0034